To investigate whether the thermal environment has impact on hemorheology characteristics of living creature, feed the experimental specimen that is twelve Guinea pigs in the ventilation installation for ten days. The air’s temperature, relative humidity and ventilation mode in the ventilation installation can be controlled. Sample the blood from the hearts of Guinea pigs and then test the hemorheology characteristics of it. Do significance analysito the data. The result shows that thermal environment has impact on hemorheology characteristics of living creature. This consequence established a basis for an indoor air quality (IAQ) evaluation method at hemorheology characteristics aspect.